Research Library
Suppression of colon carcinogenesis by bioactive compounds in grapefruit.
Authors: Vanamala J, Leonardi T, Patil BS, Taddeo SS, Murphy ME, Pike LM, Chapkin RS, Lupton JR, Turner ND.
Source: Carcinogenesis. 2005 Dec 29
This study evaluated the hypothesis that untreated and irradiated grapefruit as well as the isolated citrus compounds naringin and limonin would protect against azoxymethane (AOM) induced aberrant crypt foci (ACF) by suppressing proliferation and elevating apoptosis through anti-inflammatory activities. Male Sprague Dawley rats (n = 100) were provided one of five diets: control (without added grapefruit components), untreated or irradiated (300 Gy, (137)Cs) grapefruit pulp powder (13.7 g/kg), naringin (200 mg/kg) or limonin (200 mg/kg). Rats were injected with saline or AOM (15 mg/kg) during the 3(rd) and 4(th) wk and colons were resected (6 wk post 2(nd) injection) for evaluation of ACF, proliferation, apoptosis, and COX-2 and iNOS protein levels. Experimental diets had no effect on the variables measured in saline injected rats. However, in AOM-injected rats, the experimental diets suppressed (P